Earning Minimum Wage as a Single Parent Essays

Comcept Analysis Topic Compliance in Nursing and allied Healthcare Essay

Comcept Analysis Topic Compliance in Nursing and partnered Healthcare - Essay Example It can't be denied that protected drug is a piece of the patient's functional recuperating process. In lieu of this, do nurture truly need to know about the procedures of self medicine with the goal that they also can inevitably share the data to their future patients Attendants include the biggest single part of emergency clinic staff, they are the essential suppliers of medical clinic tolerant consideration, and they convey the greater part of the country's drawn out consideration. Most human services administrations include some type of care by medical caretakers. Albeit 60 percent of all utilized Registered Nurses (RNs) work in medical clinics, many are utilized in a wide scope of different settings, including private practices, general wellbeing offices, essential consideration facilities, home medicinal services, outpatient surgicenters, wellbeing support associations, nursing-school-worked nursing focuses, protection and oversaw care organizations, nursing homes, schools, psychological well-being offices, hospices, the military, and industry. Different attendants work in vocations as school and college teachers getting ready future medical caretakers or as researchers creating progresses in numerous regions of medicinal services and wellbei ng advancement (http://nursing.about.com/gi/dynamic/offsite.htmsite=http://www.aacn.nche.edu/instruction/Career.htm, 2004). With these exceptionally unique elements of attendants, it is then got basic for them to gain extra abilities and information that would help them in the fruitful fulfillment of all the medical attendant's shared objective, thus, the sheltered prescription administration ought to be conferred as extra aptitudes for attendants. The sum total of what attendants have been educated with the five privileges of prescription organization. The correct patient, the correct medication, the correct portion, the correct daily schedule and the opportune time are the very establishment from which attendants practice securely while administrating drugs to the patients in any social insurance setting (http://www.lklnd.usf.edu/Colleges/Nursing/nursing.html, 2005). Similarly as attendants know the five privileges of prescription organization, they ought to likewise know the sheltered medicine the board methods, which will clearly control nurture as they keep on thinking about patients regardless of these violent occasions. (http://www.lklnd.usf.edu/Colleges/Nursing/nursing.html, 2005). The six security prescription administration rehearses are as per the following: Complete and Clearly Written Order Any attendant should realize that they ought to consistently make sure that they have request which is finished and plainly composed. They reserve the privilege to necessitate that the medication, portion, course and recurrence be composed by the doctor. These segments must be available for a doctor request to be viewed as complete (http://www.lklnd.usf.edu/Colleges/Nursing/nursing.html, 2005). Right Drug Route and Dose Dispensed Attendants oversee drugs yet it is the drug store's obligation to apportion meds accurately. A suggestion from the Massachusetts Hospital Coalition expresses that a unit portion arrangement of drug can diminish the quantity of medicine blunders. Numerous emergency clinics have embraced this arrangement of medicine organization (http://www.lklnd.usf.edu/Colleges/Nursing/nursing.html, 2005). Access to Information Medical caretakers ought to be refreshed and have a simple open medication data. This implies the emergency clinic model, a Physicians Desk Reference and a present nursing drug reference book need

Is Domestic Discipline Abuse or Loving Correction

Is Domestic Discipline Abuse or Loving Correction Relationships Spouses & Partners Marital Problems Print Is Domestic Discipline Loving Correction or Domestic Violence? By Sheri Stritof Sheri Stritof has written about marriage and relationships for 20 years. Shes the co-author of The Everything Great Marriage Book. Learn about our editorial policy Sheri Stritof Reviewed by Reviewed by Amy Morin, LCSW on February 01, 2020 facebook twitter instagram Amy Morin, LCSW, is a psychotherapist, author of the bestselling book 13 Things Mentally Strong People Dont Do, and a highly sought-after speaker. Learn about our Wellness Board Amy Morin, LCSW on February 01, 2020 SolStock / Getty Images More in Relationships Spouses & Partners Marital Problems LGBTQ Violence and Abuse The domestic discipline (DD) movement encourages wife-spanking as discipline and is condoned and supported by some religions and religious leaders. But many argue that allowing husbands this type of authority in a marriage can lead to or easily become spousal abuse and can destroy the self-esteem and dignity of the person being spanked.?? Heres a look at how the practice borders on domestic abuse, if it constitutes violence, and whether its scripture-based or sexually motivated.?? What Is Domestic Discipline? Domestic discipline, or Christian domestic discipline, is a submissive heterosexual marriage lifestyle that encourages husbands (who are considered the head of the household or HoH) to spank their wives for mistakes or misbehavior.  There may be other methods of punishment used as well, including time-outs, loss of driving privileges, etc.?? Those who support the domestic discipline lifestyle give their reasons, which are often at odds with what research says about the practice. What Supporters Say It comes from the Bible. It is not BDSM (bondage, discipline, dominance, submission, sadomasochism). It is discipline; it is non-erotic. It is an essential part of the marital relationship. It provides a physical correction with one who truly cares. It is responsible authority. It is appropriate punishment. It requires total consent from both parties. What Research Says It is based on misinterpretations of the Bible. It is a version of BDSM. It may be sexually erotic. It is not life-giving to relationships. It is a control issue and is potentially abusive. Marriage calls a couple to mutuality, not superiority of one  spouse  over the other that involves punishment.   Is Domestic Discipline Scripture-Based?   Although defenders of domestic discipline believe that the DD lifestyle is based on Bible passages, some Christian religious leaders disagree. Those who practice domestic discipline often mention scripture texts that call for a woman to be submissive to her husband, but there are many who follow a submissive marriage belief without beating or striking their wives. The difficulty with the traditional view of headship is that it has been misused to keep women subservient and in some cases to justify the emotional and/or physical abuse of women within the couple relationship, The Convention of Atlantic Baptist Churches states in Harmony in the Home. This is far from mutual submission and is not as God intended for the couple relationship.   I do not believe that there is one husband who is so perfect that he would be justified to use that kind of authority, said Reverend Al Blonigen, Chaplain for the metro-Detroit Retrouvaille, in reference to spanking. Besides, nowhere in Scripture did Jesus use physical pain on anyone. In the New Testament, Paul, the writer of Colossians, offers instructions for Christian households with a particular emphasis on wives submitting to their husband, notes Lisa Bahar, MA, CCJP, LMFT, LPCC, a licensed marriage and family therapist and professor of psychology at Pepperdine University, a Christian university in California.   My understanding of this scripture is that wives would follow their husbands leadership in Christ, says Dr. Bahar. Just as Christ served his disciples to the point of washing their feet, he is asking husbands to serve their wives. A wise and Christ-honoring husband will not abuse his wife, which would include a physical altercation like spanking.     Why Do Some Women Agree to Domestic Discipline? Some believe that women want or agree with domestic discipline due to guilt over past sexual behavior, or because they believe that God has deemed that disciplinarian is the proper role of a husband. DD may be appealing to women who want to be free from having to make decisions or taking responsibility in life. Some women may believe DD gives them free rein to do what they want and being spanked or losing driving privileges eliminates the wrong. But according to some anti-DD Christian groups, the woman is relegated to child status with little motivation to grow into a mature woman of God. Some women may deem these acts of discipline as acceptable or part of their subculture due to a history of sexual or physical abuse, notes Dr. Bahar. Others may find spanking sexually exciting. There may be an adrenaline rush combined with the sexual arousal and this chemical reaction can be addictive, explains Dr. Bahar. Domestic Violence Within the Domestic Discipline Lifestyle Dr. Bahar says DD can be considered a form of domestic violence or intimate partner violence, as it is consistent with the three phases of domestic abuse as conceptualized in the late 1970s by psychologist  Lenore Walker: Tension-building phase (the build-up to domestic abuse)Acute battering episode (the spanking)Honeymoon phase (possible remorse, pursuit, and denial of the severity) She adds that DD is a physical, mental, emotional, and possibly sexual safety risk of health and well-being.  And it can be even more damaging if children observe these acts of violence. A Word From Verywell According to the National Coalition Against Domestic Violence, one in three U.S. women has been the victim of domestic violence in an intimate relationship. Almost 20 people of any gender are abused by a partner every minute.?? If you are in a domestic discipline marriage and are worried about the safety of yourself and your children, dont wait to get help. Turning to a marriage or mental health counselor can help you break the silence and make an informed, rational decision about the health of your relationship. The Best Online Marriage Counseling Programs

The United Republic Of Haiti - 704 Words

English is the most broadly spoken language worldwide. However, there are many non-English speaking countries in the Americas and the Caribbean. Haiti is one of the non- English speaking Caribbean countries. Their way of life of a group of people developed over time and passed down from generation to generation. The Republic of Haiti is located in the Caribbean Sea, between Cuba and Puerto- Rico. It is a tropical country with an average temperature of 80 degrees Fahrenheit. The capital of Haiti is Port-au-Prince and its official languages are French and Creole. It would be impossible to cover every aspect of Haitian culture in a short cultural paper. We have therefore, chosen to take a values approach to discuss power, prestige, filial responsibility, modernization, status and roles of older adults in that culture. Power Family power is significant to understand how families functions as a unit. It helps the family to make important decisions about how to manage their lives, whether it financially, about where to live, educational choices, or even about leisure time, and so on. In Haiti, women play a major role in the culture. They referred to them as the â€Å"Central Pillar† They endure most of the burden of childcare, making sure that the children are well fed and healthy, and keep the house cleaned. They also work in the agriculture. They cultivated goods, carried them in their head, and walked miles to sell at the supermarket to pay for their children’s schoolShow MoreRelatedBlack in Latin America688 Words   |  3 PagesLatin America: Haiti and the dominican Republic 1) How did Haiti and the Dominican Republic begin? What European countries influenced the development of these nations? - Haiti and the Dominican Republic is an island with two countries of Hispaniola. Haiti makes up 1/3 of the island while the Dominican Republic makes up the rest of the 2/3 of the island. The Dominican Republic contains a mix blood of Afro-European population with the history of Spanish colonialism, while Haiti has African-caribbeanRead MoreChristopher Columbus And The New World1579 Words   |  7 Pagesfirst ceded the western third of the island (now Haiti) and later on the other two thirds (Dominican Republic). Haiti eventually had a revolution and became independent from the French, but because of the strong Spanish influence, Dominican Republic became independent from Haiti later on. Although the French were now in control of the whole island, the eastern two thirds still maintained a strong Spanish influence. Although Haiti and Dominican Republic are on the same island and one might think bothRead MoreDominican Republic vs. Haiti1245 Words   |  5 PagesDominican Republic and Haiti: A Comparison of How One Island Has Two Complete Different Ends Dominican Republic and Haiti: A Comparison of How One Island Has Two Complete Different Ends The Hispaniola is a small island in the Caribbean which is currently composed by the Dominican Republic and the Republic of Haiti. These two countries despite sharing an island and historical institutions are completely different. While the Dominican Republic enjoys the beauty of the Caribbean and its weatherRead MoreResearch on Haiti Essay1156 Words   |  5 PagesHAITI: IT’S PAST AND PRESENT Haiti is the second largest Caribbean Island. It occupies a third of the western part of the island it shares with the Dominican Republic. Haiti is also made up of several islands that surround the main territory. The capital is Port-au-Prince. It rains between November and March in the North of the island and between May and October in the South. â€Å"Once covered by forest, the country has been heavily logged for wood and fuel and to clear land for farming, and isRead MoreDominican Republic : The Island Of The Country1123 Words   |  5 PagesDominican Republic was formally known as the island of â€Å"Hispaniola† named by Christopher Columbus. The mountainous country currently shares itself with Haiti. On February 27th, 1844, Dominican Republic gained independence from war against Haiti. Although the country has faced many hardships from the incoming decades, the country has survived. The country is located between the Caribbean Sea and the North Atlantic Ocean, and east of Haiti. The capitalRead MoreHaiti : The Country Of The Western Hemisphere1109 Words   |  5 Pages Haiti, located in the western, smaller portion of the island Hispaniola, is about the size of Maryland. It’s in the Greater Antillean archipelago, which is shared with the Dominican Republic. There are three different languages spoken there , Haitian, Creole, and French. Haiti is known as the poorest country in the Western Hemisphere, and in the midst of poorest in the world. With a population of approximately nine hundred thousand people, after Cuba, it is the second most populous country in theRead MoreHaiti And The Dominican Republic Essay1581 Words   |  7 PagesIntroduction The caribbean is where a small island, contains two countries within its borders and a long history of conflict. Haiti and the Dominican Republic are two different country, with completely different cultures, from religion, to the food they eat, Two countries, with two different, yet correlating stories. A story of conflict is what unites these two countries. This paper will attempt to analyse that story, and answer how this small island came to be divided into two countries, and inhabitedRead MoreThe Culture Of The Dominican Republic995 Words   |  4 PagesHaiti, the small country located west of the Dominican Republic, is a unique and beautiful country with its own unique culture and geography. The people of Haiti are proud of their country and their culture. Even though the nation is plagued by earthquakes and storms, due to its geographic location, it does not stop the Haitian people from continuing their unique culture and happy lifestyle. Taking a look at this countryâ€⠄¢s culture and geography, anyone, civilian, military or political personnel canRead MoreThe Millennium Development Goals : A Global Vision For The Future1447 Words   |  6 PagesAbstract On September 8, 2000, the United Nations Millennium Summit concluded with the adoption of the Millennium Declaration as a global vision for the future. In the following years, the Millennium Development Goals (MDGs) evolved in an effort to save the Declaration from slipping into oblivion. They created a momentum that brought the issue of development back on the international agenda, overcame aid fatigue and galvanized the public. With the MDGs’ expiration date of 2015 fast approaching, theRead MoreFrench and Hatian Revolutions Similarities and Differences941 Words   |  4 PagesBetween 1750 and 1870, countries such as France and Haiti experienced dramatic economic, political, and social changes through Revolutions. A revolution occurs when change is desired by people who were mistreated and for this reason, the French and Haitian revolution occurred, leading to many different governmental changes such as the first republic of France and the rule of Napoleon, and the free rep ublic and independent nation established by Haiti. They also led to many similar government changes

Essay on The American Dream in Citizen Kane and The Great...

The United States of America is the most powerful and wealthy country in the world. The varieties of class, individuality, religion, and race are a few of the enrichments within the quot;melting potquot; of our society. The blend of these numerous diversities is the crucial ingredient to our modern nation. Even though America has been formed upon these diversities, its inhabitants- the quot;average Americanquot;- have a single thing in common; a single idea; a single goal; the American Dream. The Dream consists of a seemingly simple concept; success. Americans dream of a successful marriage, family, successful job, and own a Victorian-style home with a white picket fence and an oak tree with a swing tire in the front yard. The†¦show more content†¦In responses to the letter sent to Charles offering numerous businesses to own, he writes his disinterest in all of the quot;sure-moneyquot; businesses except the New York Inquirer. quot;I think it would be fun to run a newspaper.q uot; Charles absence of seriousness in the awareness of the gross profit conveys his carelessness about money. Instead of running a mining company and gaining a definite profit, he chooses to run the Inquirer because it would be quot;fun.quot; Charles conveys his carefree emotions about his money and concentrates more on his own personal enjoyment. quot;At a million dollars a year, Ill have to retire in... sixty years.quot; Charles snickers at the rate of his money loss and again he shows no interest in his mass money, his only interest is in keeping himself busy and happy (something he cannot maintain). quot;So were bust. Just give me the paper so I can sign it and go home.quot; Even after learning that the Inquirer had to be shut down because of lack of money, Charles signs the paper as if its only value was his ticket home. nbsp;nbsp;nbsp;nbsp;nbsp;Throughout the reporters interview with Mr. Bernstein, many clues to the quot;Rosebudquot; mysteryShow MoreRelatedEssay on Kane, Gatsby, And The American Dream1035 Words   |  5 PagesThe Great Gatsby written by F. Scott Fitzgerald and Citizen Kane a movie directed by Orson Welles are both monumental stories in American society as they both represent the American dream at it’s most brilliant high. The Great Gatsby is all about time and the American dream; it is essentially what consumes Gatsby. Both Kane and Gatsby are representations of the American dream, and as we read into their stories we see that time and the dream become so intertwined that it is hard to see them apartRead MoreGreat Gatsby and Citizen Kane compare and contrast Essay examples677 Words   |  3 Pages The Great Gatsby Citizen Kane You are today where your thoughts have brought you; you will be tomorrow where your thoughts take you. - James Allen The Great Gatsby and Citizen Kane are both classic American stories about the so called â€Å"American Dream†. The main characters in both stories are Mr. Gatsby and Mr. Kane. They each come from similar backgrounds. They also both hold much power as adults. A difference between the two is one chose their way of living the other did not. He wasRead MoreGatsby and the Complexity of the American Dream4080 Words   |  17 PagesGatsby and the Complexity of the American Dream F. Scott Fitzgerald’s The Great Gatsby is an exploration of the American dream in modern society, in which money and prosperity are significant factors and it may not be as simple as you think; and the movie Citizen Kane is another example of the complex issues relating to the American dream. First, we will explore the American dream, in which it is to make a great deal of money because it provides for a comfortable living, and characters in the novel

Food Microbiology Free Essays

string(280) " reason why microbial cells are highly sensitive to allicin is probably because the lack of glutathione \(thiol molecules such as trypanothione\) which results of lack of the ability to reactivate the pivotal SH-enzymes that are thiolated by allicin \(Ankri and Mirelman, 1999\)\." CHAPTER I INTRODUCTION 1. 1 Background Food is one of human sources of calorie, protein, fats, and nutrition. Yet, because of the highly nutritious content, food is susceptible to growth of microorganisms. We will write a custom essay sample on Food Microbiology or any similar topic only for you Order Now By the presence of microorganisms in food, the food is more likely to have shorter shelf life. Thus, mostly it is resolved by the addition of antimicrobial substances to food, such as condiments and preservatives. Condiments and preservatives could inhibit the growth of microorganisms or even destroyed them, as they have antimicrobial agents. Some examples of condiments and preservatives are ginger, clove, sodium benzoate, garlic, and coriander. The factors contributing in the effectiveness of condiments and preservatives in inhibiting the growth of microorganisms are the concentration of the antimicrobial, temperature, characteristic of the microorganisms and food, storage time. As different types of microorganisms have different resistance toward the antimicrobial substance, it is important to understand the characteristic of the microorganisms towards the antimicrobial agent. There are two types of resistance: intrinsic resistance and acquired resistance. There are some methods of observing the capability of the condiments in inhibiting the growth of microorganisms. In the experiment, the method used is well diffusion method, which used different type of condiments and added into holes of agar, where by the inhibition zone by the condiments could be observed. The larger the inhibition zone, the more effective the condiment was in inhibiting the growth of microorganisms. 1. 2 Objectives By conducting the experiment, students are expected to learn and observe he effectiveness of antimicrobial substance consisted in condiments and potassium sorbate towards the growth of microorganisms. CHAPTER II LITERATURE REVIEW 2. 1 Antimicrobials Referring to Volk and Wheeler (1993), antimicrobials are the substances that are used to inhibit or kill pathogenic or non-pathogenic microbes. Antimicrobials are also often used as sanitizers and preservatives. Sanitizer is an agent to decrease the amount of microorganisms to the acceptable level. It is generally used in food processing equipments. Antimicrobials are grouped into natural antimicrobials and chemical antimicrobials. According to Fardiaz (1992), antimicrobials may be microstatic, which is inhibitory to the growth of microbes, and microcidal, which means able to kill microbes. Fruit extract is one most susceptible food materials and thus, is often added with preservatives, especially chemical preservatives, stored at low temperature, or pasteurized. Pasteurization is a heating process at 63? C for thirty minutes. This process is aimed to preserve the stability of the food materials (Buckle et al. , 1987). The growth of microbes can be controlled by using various methods, namely physical method, chemical method, and immunological method. The control of microorganism growth is performed to kill the microbes, to inhibit the microbes, and to destroy the microbes. Physical controls can be performed by sterilization with heating or radiation and filtering. Chemical controls can be carried out by using chemical antimicrobial compounds, such as disinfectants and preservatives. Whereas immunological controls can be performed by vaccination (Batzing, 2002). 2. 2 Active Antimicrobial Compounds in Spices 2. 2. 1 Garlic Garlic come from the onion family and are an erect biennial herb, which grow annually. It has irregular roots, condensed, flattened step and narrow and has lat leaves. Garlic’s bulb consists of 6 to 35 bulblets called cloves which enclosed in a thick whitish, glistening, and transparent covering (Anonym1, 2000). According to Ankri and Mirelman (1999), garlic or Allium sativum or lahsoon in Indian name is an edible plant, which has been generating a lot of interest as a medicinal panacea and a cure for a wi de variety of different conditions since the human history has begun. It is reported to have anticancer effects and to reduce blood lipids in human body. Figure 2. 1 shows the approximate composition of fresh garlic. Figure 2. The approximate composition of fresh garlic Source: Ahmad (1996) The active compound found in garlic cloves which have an unusual concentration of sulfur-containing compounds (1-3%) is called allicin. It is a volatile molecule, which is poorly miscible in aqueous solution, and has a strong typical odor of crushed garlic. Chemically, allicin can be synthesized by mild oxidation of diallyl disulfide as presented in Figure 2. 1. It is to be noticed in Figure 2. 2 that there is a compound called alliin which is a stable precusor, that later will be converted to allicin by enzyme called alliinase present in cloves too. Moreover, alliinase is surprisingly found in large amounts in cloves, which is about 10% of the total protein content. Practically, allicin is produced when garlic cloves are cut into or crushed (Ankri and Mirelman, 1999) Figure 2. 2 Generation of allicin in a garlic clove Source: Ankri and Mirelman (1999) According to Ankri and Mirelman (1999), there are several biological activities in allicin such as its activity as an antioxidant and its ability to attack the sulphur (SH) groups in enzymes and proteins while modifying their activities as well. Furthermore, allicin can rapidly penetrate into cells through the cell membranes. In its pure form, allicin has been reputed to exhibit antibacterial activity against a wide range of Gram-negative and Gram-positive bacteria, for instance Escherichia coli that is known to be a multidrug-resistant enterotoxicogenic strains, Salmonella, Staphylococcus, Streptococcus, Klebsiella, Proteus, Bacillus, and Clostridium. It also has antifungal activity that prevents the formation of mycotoxins such as the aflatoxin of Aspergillus parasiticus. Allicin has shown the anticandida activity towards and is effective against the group species of Candida, Cryptococcus, Trichophyton, Epidermophyton, and Microsporum at only low concentration since it inhibits both the germination of spores and the formation of hyphae. Referring to Dobre et al. (2011), allicin can also attack Aspergillus, Fusarium, and Penecillium species, which are responsible for food poisoning and food decay. The main mechanism of the antimicrobial activity in allicin is the inhibition of certain thiol-containing enzymes in the microorganisms by the super fast reaction of thiosulfinates with thiol groups, such as alcohol dehydrogenase, thioredoxin reductase, and RNA polymerase that later will affect the essential metabolism of cysteine proteinase activity. The reason why microbial cells are highly sensitive to allicin is probably because the lack of glutathione (thiol molecules such as trypanothione) which results of lack of the ability to reactivate the pivotal SH-enzymes that are thiolated by allicin (Ankri and Mirelman, 1999). You read "Food Microbiology" in category "Essay examples" . 2. 2 Coriander Coriander or Coriandrum sativum L. is originated from the Mediterranean region and has the appearance of flat shape in the one side while slightly pointed shape is found in the other side. Coriander seed has various lengths between 3 to 5 mm with brown color in ripe state (Sarkar, 2012). A ccodring to Rattanachaikunsopon and Phumkhachorn (2010), it has been traditionally used as an analgesic, aphrodisiac, antirheumatic, anti-inflammatory, diuretic, antispasmodic, circulatory stimulant, and antidiabetic. Beside that, coriander is known to have effect in lowering cholesterol. Table 2. shows the composition of coriander seed. Table 2. 3 The coriander seed composition in 1 tsp Total Fat 0. 9g 1% Saturated Fat 0. 00g 0% Monounsaturated Fat 0. 7g Polyunsaturated Fat 0. 1g Cholesterol 0mg 0% Sodium 2mg 0% Total Carbohydrates 2. 7g 1% Dietary Fiber 2. 1 g 8% Protein 0. 6g Vitamin A 0% Vitamin C 2% Calcium 4% Iron 5% Source: Sarkar (2012) The precise volatile compounds acting as antimicrobial compounds have not been examined clearly till now, although there are some volatile compounds suggested to be the antimicrobial compound inside coriander which are (2E) – hexenal and (3E) – hexenal (Kubo et al. , 2004). They are reported to have antimicrobial activity against Gram-negative and Gram-positive bacteria such as Escherichia coli O157:H7, Listeria monocytogenes, and Staphylococcus aureus, which are foodborne pathogenic bacteria. It also exhibits bactericidal activity and is reported to have an effective antibacterial. The mechanism of the activity of coriander is membrane damage causing cell death to the bacteria (Silva et al. , 2011). However, according to Uma et al. (2009), coriander seems to not having an effective antifungal activity and its activity toward yeast have not yet been examined further. 2. 2. 3 Black Pepper Black pepper (Pipper nigrum) is a condiment that has been used since ancient times and is native to India. Black pepper is useful for treatment of various sicknesses such as vertigo, asthma, fever and also cholera. The volatile oil of black pepper has been shown to have antimicrobial activity as well (Karsha and Lakshmi, 2009). The major antimicrobial compund found in black pepper are monoterpenes and sesquiterpenes (Davidson et al. , 2005). According to Karsha and Lakshmi (2009), black pepper shows strong antimicrobial activity against gram-positive bacteria such as Staphylococcus aureus, Bacillus cereus and Streptococcus faecalis. Gram-negative bacteria such as Pseudomonas aeruginosa, Salmonella typhi and Escherichia coli are affected as well, although the effect on gram-positive bacteria is better. The mechanism of the antimicrobial activity appears to be loss of control over cell membrane permeability. According to Singh et al. (2004), black pepper has antifungal activity as well as it is effective in stopping the growth of molds such as Fusarium graminearum. Black pepper is also shown to be able to inhibit the growth of yeast such as Candida albicans (Joe et al. , 2009). 2. 2. 4 Potassium Sorbate Potassium sorbate are currently one of the most widely used preservative and can be used to preserve foods, animal feeds, pharmaceuticals, and cosmetics. Potassium sorbate may be manufactured as a powder or granules and has an antimicrobial potency of 74% compared to sorbic acid. The molecular weight of potassium sorbate is 150. 22 and is the most soluble form of sorbate compared to the others, such as calcium sorbate and sodium sorbate. Besides good solubility, potassium sorbate is also has good stability and easy to manufacture, making it the most used form of sorbate in food industry. Sorbate is very effective when used against bacteria, molds, and yeasts. Yeasts inhibited by sorbate are Brettanomyces, Candida, Cryptococccus, Debaryomyces, Endomycopsis, Hansenula, Kloeckera, Pichia, Rhodotorula, Saccharomyces, Sporobolomyces, Torulaspora, Torulopsis, and Zygosaccharomyces. Molds species inhibited by sorbate are Alternaria, Ascochyta, Ascosphaera, Aspergillus, Botrytis, Cephalosporium, Chaetomium, Cladosporium, Colletotrichum, Cunninghamella, Curvularia, Fusarium, Geotrichum, Gliocladium, Helminthosporium, Heterosporium, Humicola, Monilia, Mucor, Penicillium, Phoma, Pepularia, Pestalotiopsis, Pullularia, Rhizoctonia, Rhizopus, Rosellinia, Sporotrichum, Trichoderma, Truncatella, Ulocladium, and others. While for bacteria, the species inhibited are Acetobacter, Achromobacter, Acinetobacter, Enterobacter, Aeromonas, Alcaligenes, Alteromonas, Arthrobacter, Bacillus, Campylobacter, Clostridium, Escherichia, Klebsiella, Lactobacillus, Micrococcus, Moraxella, Mycobacterium, Pediococcus, Proteus, Pseudomonas, Salmonella, Serratia, Staphylococcus, Vibrio, Yersinia, and others (Davidson et al. , 2005). 2. 3 Mechanism of Antimicrobials There exist some mechanisms of antimicrobial activity in inhibiting the growth of microbes. The antimicrobials are classified into: cell wall destructor, cell wall permeability intervention, destructor of proteins, nucleic acids, and enzymes, antimetabolites, inhibitors of nucleic acid synthesis, and cell plasmolysis (Fardiaz and Betty, 1989). Referring to Fardiaz and Betty (1989), the broken cell wall of microorganisms will cause the cell content to depart from the cell and thus, inhibit the cell metabolisms. Severe destructions on the cell may cause the death of the cell. Lysozyme is one of the enzymes that are able to destruct the cell wall of Gram-positive bacteria. An enzyme produced by a bacterium might be able to inhibit the growth of other microbes. There is also a type of antimicrobials that is able to inhibit the formation of cell wall materials. These cells that do not have cell wall are called protoplast. Protoplast is very easily broken, except when placed in isotonic medium. Penicillin and cycloserin are examples of compounds that retard the formation of peptidoglycan in developing cell. Gram-positive bacteria are susceptible to penicillin as they have lots of peptidoglycans. The breaking of plasma membrane and leakage of cell content will inhibit the microorganism growth or even kill the microorganisms. The implication if the cell destruction is the enzymes will not be able to function properly in the cell metabolism. However, antimicrobials that intervene the cell wall permeability are rarely used in food industries (Fardiaz and Betty, 1989). Compounds destructing the protein and nucleic acid are able to destroy cells. This type of destruction is unfixable. For instance, certain amount of alcohol and sodium chloride are able to denaturate the proteins. Those two compounds are often used in food industries (Fardiaz and Betty, 1989). Antimetabolites are the compounds that are similar to natural metabolites. These antimetabolites will interrupt the metabolisms in the cell. The interruption of the metabolisms might retard the cell growth or even kill the cell. Furthermore, the synthesis of DNA and RNA may be inhibited by some antimicrobial compounds, namely the compounds that are able to retard the formation of nucleic acid arrangement and compounds that inhibit the nucleic acid polymerization (Fardiaz and Betty, 1989). Plasmolysis or the breakage of a cell is caused by the high plasmolysis pressure. Materials that are often used in food to promote the cell plasmolysis are salt and sugar. Salt and sugar are considered to be able to increase the osmotic pressure in food materials therefore plasmolysis takes place (Fardiaz and Betty, 1989). 2. 4 Antimicrobial Sensitivity towards Microbial Defense Microorganisms have the ability to resist some types of antimicrobial substances. There are two types of resistance in microorganisms toward antimicrobial agents, such as intrinsic or natural resistance and acquired resistance. The antimicrobial agents in intrinsic resistance could not affect the microorganisms, as they have no target sites, which are the microorganisms, to affect. In contrary, in microorganisms that don’t have the intrinsic resistance, the antimicrobial substance could gradually enter the microbial cell and affecting the activity of the microorganisms, as the microorganisms’ cell membrane have lower permeability to antimicrobial substance (Sosa et al. , 2010). Furthermore, in acquired resistance, the microorganisms are naturally vulnerable, as they need specific ways for preventing to be affected by the antimicrobial substances. Some examples of the specific ways are the presence of enzyme that has the ability to inactivate the antimicrobial agent, or alternative enzyme that has the ability to inhibit the activity of antimicrobial agent. Then, it also happens when there is mutation and post-transcriptional and posttranslational in the microorganisms that are the target of the antimicrobial agent. Thus, these will reduce the binding of the antimicrobial agent (Sosa et al. , 2010). Every microorganism has different sensitivity towards different type antimicrobial agents. Coriander (Coriandrum sativum L. is mostly used as seasoning condiment. According to Kubo et al. (2004), in the leaves of coriander, there are volatile oils that are suggested to have antimicrobial properties against food born pathogen, such as Salmonella species, which are gram-negative bacteria. Black pepper (Piper nigrum L. ) is used mostly in food as seasoning condiments. The aqueous and ethanolic extract of black pepper is very effective for inhibiting antibacterial activity agains penicillin G resistant strain of Staphylococcus aureus, Bacillus cereus and Bacillus subtilis (Chaudhry and Tariq, 2006). According to Karsha and Lakshmi (2010) experiment, gram-positive bacteria were more susceptible towards antimicrobial in black pepper than gramnegative bacteria. In gram-positive bacteria, the most susceptible towards antimicrobial in black pepper is Staphylococcus and followed by Bacillus and Streptococcus. Furthermore, among gram-negative bacteria, the most susceptible towards antimicrobial in black pepper is Pseudomonas, followed by E. coli, Klebsiella and Salmonella. Garlic (Allium sativum) is commonly used for antifungal, antiviral, antibacterial, antihelmantic, antiseptic and anti-infamatory. Garlic extract is effevtive in inhibiting the microbial activity of both gram-positive and gramnegative bacteria. Several examples of the gram-negative bacteria are E. coli, Salmonella species and Citrobacter Enterobacter, Pseudomon, Kilabsella) and the gram-positive bacteria are Pseudomonas aeruginosa, Salmonella typhi, Proteus, spp. , Staphylococcus aureus, S. pneumonia Group A streptococcus and Bacillus anthrax (Daka, 2011; Durairaj, 2009). Potassium sorbate is used as preservative in food as the sorbic acid is more effective than benzoic acid in preserving food. The preservation process occurs in higher pH. It is effective to inhibit the microbial activity of Pseudomonas species, which are categorized as gram-positive bacteria (Beuchat, 1980). Mostly, gram-positive bacteria are more sensitive towards antimicrobial agent than gram-negative bacteria (Torrence and Isaacson, 2003). 2. 5 Factors Affecting Microorganism Strength towards Antimicrobial Compounds The resistance of microbes towards particular antimicrobial compound is dependent on several factors that contained in that particular microbe such as the cell wall, protein content, nucleic acid, and membrane cell (Lay, 2002). There are several types of microbes that have cell walls, and an antimicrobial compound that can affect the mechanism of the cell wall that influence the microbial resistance. Anti-microbial compounds may interfere with the work of the cell wall as well as peptidoglycan biosynthesis, which is the process in prokaryotic cell wall structure construction. Disruption of peptidoglycan may affect the resistance and sensitivity of microbes to changes in osmotic perspective (Lay, 2002). Microbes also contain nucleic acids. The nucleic acid can be affected by antimicrobial compunds where the enzymes that are going to be used for the synthesis of nucleic acid are inhibited. The example is rifampin where it binds the enzyme RNA polymerase, quinolone as well as binding enzyme DNA gyrase (Lay, 2002). The resistance of microbes can be affected by the metabolism of the microbes. Enzyme that catalyzes the synthesis of essential molecules can be inhibited by antimetabolite. Antimetabolite is the antimicrobial that is used for inhibiting the growth of microbes. An example of antimetabolite is the sulfinylamide (Lay, 2002). In general, most living cells including microorganisms has the ability to control what is going in and out of the membrane. If there is a rupture in the membrane, it will cause the spillage of the essential inorganic ions. Thus, rupture in the cell membrane can affect the growth and death of the microbes (Lay, 2002). 2. 6 Methods of Microbial Defense towards Antimicrobial Activity 2. 6. 1 Well Diffusion Method Well diffusion method is done by pouring the microbial suspension that is going to be tested into a sterile Petri dish continued by pouring the media agar into the dish. Then, the Petri dishes are mixed by the eight movement ethod to allow even mixing. The media is then allowed to be solidified and cynlidrical holes are made. The holes are then filled with antimicrobial agents to be tested. The cup was incubated at 37Â °C for two days and then observed on the antimicrobial activity of the tested microbial suspension. Antimicrobial ingredients poured into the well are able to s pread evenly as it can diffuse in all directions around the microbial suspension. Once incubated, usually will form a clean circular zone of microbes. This method can be used to test several different types of antimicrobial agents. (Smith, 2005) 2. 6. Kirby Bauer Disc Method One of the easy methods to test the vulnerability of organisms towards the antimicrobial agents is by inoculating the agar with culture and allowing the antimicrobes to diffuse to the media agar. The discs that contain antimicrobial agents are placed on the surface of the plate that has the organisms that are going to be tested. At particular distance on respective discs, the antimicrobes will diffuse to a point where the antimicrobes are unable to inhibit the microbial growth. Antimicrobial effectiveness is shown by the inhibition zones. Inhibition zone appears as clean areas that surround the disc. The diameter zone can be measured by using ruler and the result of the experiment considered to be one antibiogram. (Smith, 2005) 2. 6. 3 MIC and MBC The antimicrobial activity can be observed by knowing the concentration of the antimicrobial agents that are going to be used by reducing the total critical number of bacteria that caused their death. MIC (minimum inhibitor concentration) from an antimicrobes can be known by providing the antimicrobial agents into two serial dilutions in series tubes or can be done by well method in a media that has been inoculated with bacteria. Series tubes that have been filled with antimicrobial agents are incubated to see the growth of the bacteria and the turbidity is observed. The increment in turbidity indicates the growth of the microbes. MIC is the lowest concentration of an antimicrobial agent where the grwoth can be inhibited completely. (Smith, 2005) MBC (minimum bactericidal concentration) is the total number of antimicrobial agents that is required to kill organisms. MBC is done by taking a part from each serial tubes from the MIC that does not show any growth of the bacteria that has been incubated, The samples is taken from respective tubes that has been incubated in pour plate media. MBC is the lowest concentration of antimicrobial agents that are able to kill at least 99. 9% of the inoculums that has been incubated (Smith, 2005). 2. 7 Food Preservatives Preservatives are commonly used to prevent destruction of physical, chemical and microbiological food. Use of antimicrobial preservatives combined with other preservatives so that the preservation of the food will be maximized. According Fellows (2000), the criteria for antimicrobial preservative in food are the usage and is more efficient which when dissolved in water, stable in storage, non-toxic, low antimicrobial concentrations but has a wide range, optimum efficiency at room temperature, non-corrosive, odorless, and its high penetrative ability to the food. Based on its mechanism, the antimicrobial agent acts as the rupture of the cell wall When there is a rupture on the cell wall, the cell contents will spill out thus inhibiting the metabolism of cells. The destruction of the cell wall can result in cell death. Lysozyme can damage the cell walls of Gram-positive bacteria. Penicillin and sikloserin inhibit the growth of peptidoglycan in the cell develops. Gram-positive bacteria is sensitive towards penicillin as the high level of peptidoglycan content. Damage to the cell wall would also cause damage to the plasma membrane (Fellows, 2000). NaCl and alcohol is often used to preserve food because it can cause denaturation of proteins and nucleic acids that can destroy the cell and cannot be repaired. While H2O2 is an antimicrobial that can destroy the enzyme activity of microbes. Compounds inhibiting the formation of nucleic acids and nucleic acid polymerization inhibitor compounds can inhibit the synthesis of both DNA and RNA. The use of salt and sugar can cause plasmolysis the microbial cell. Since the osmotic pressure on microbial too high, so that the cells undergo plasmolisis and can inhibit the growth of microbes (Fellows, 2000). Ascorbic acid can inhibit the growth of bacteria and molds in a way to inactivate the enzyme fatty acid dehydrogenase. Ascorbic acid can work optimally at a pH above 6. 5. Propionic acid is at its most effectiveness to inhibit molds and yeasts with maximal activity at pH above 5. Acetic acid as vinegar used to preserve bread in order to prevent mold contamination, but vinegar cannot inhibit the growth of yeasts. Acetic acid work optimally at low pH (acid). Ethylene oxide and propylene oxide can be used as a fumigant in spices and flour. Sodium or potassium nitrite and nitrate are commonly used for preservatives in meat products. Maximum working power of nitrite compounds is pH of about 57. Nitrite also serves to inhibit Clostridium botulinum, Acinetobacter, Moraxella, Flavobacterium, Pseudomonas, Enterobacter, Escherichia, and some micrococcus. Nitrites are heated simultaneously with foods will provide the growth inhibitory effect against microbes ten times greater than that of nitrite without heating (Fellows, 2000). CHAPTER III MATERIALS AND METHOD 3. 1 Materials and Equipments Equipments that are used in the experiment are balance, blender, grater, dilution bottle, autoclave, waterbath, refrigerator, vernier caliper, tip with the cuttop, sterile toothpicks, sterile petri dishes, micropipette, tip (10 ml and 100Â µl), and Bunsen’s burner. Materials that are used are spices (black pepper, garlic, and coriander), potassium sorbate, alcohol, aquadest, bacteria culture (Bacillus subtilis), yeast culture (Candida tropicalis), mold culture (Aspergillus oryzae), PCA media, and NA media. 3. 2 Procedures 3. 2. 1 Extract Preparation 1. Spices were weighed up to 3 grams and reduced in size. 2. The spices were mixed with 10mL of ethanol into Erlenmeyer flasks. 3. 2. 2 Antimicrobial Assay 1. 1mL of culture is added into Petri plate 2. The matching medium is added into the Petri plates (NA for B. subtilis and PDA for A. ryzae and C. tropicalis) 3. The media were let to solidify. 4. Holes were made by using tips with cut-tops. 5. Mixtures of spices were added into the holes. 6. The plates were incubated were incubated for 48 hours in 37? C. CHAPTER IV RESULTS AND DISSCUSION 4. 1 Effect of Antimicrobial Compounds Towards Microbial Growth 4. 1. 1 Garlic Table 4. 1 Observation result of inhibitory activity by garlic From Table 4. 1, it can be seen that the microorganism that was the most susceptible to allicin contained in garlic, judging by the largest area inhibition, was Bacillus subtilis. There were even no growths in some Petri dishes that meant that the effectiveness of allicin as the antimicrobial compound could be found most when it was applied for bacteria, which was Bacillus subtilis in particular. It matched with the previous literature discussed stating that allicin is an active compound in garlic that attacks the cell membrane in microorganism. However, there was an error found in the experiment from Group 4, which resulted in no inhibition found from Bacillus subtilis. This might happen due to the absence of the allicin itself or the ontamination happened which might result in the building of the resistance towards antimicrobial compound. Afterwards, it can be seen from the experiment that Candida tropicalis and Aspergillus oryzae shared almost the same result, which means that they were also susceptible to allicin. The uneven inhibition found in Candida tropicalis showing the largest inhibition (7. 23mm) beyond the inhibition done for Bacillus subtilis migh t happen because of the uneven diffusion of the active antimicrobial compound to the dishes. It still matched the theory since allicin has antibacteria and antifungal activity towards the species mentioned above. From the result, it can be concluded that garlic can be used as the antimicrobial compound in food to prevent foodborne disease and food poisoning. However, the concentration of garlic has to be concerned since it has strong odor and may be unpleasant to some people. 4. 1. 2 Coriander Table 4. 2 Observation result of inhibitory activity by coriander From Table 4. 2, it can be seen that coriander is found to be effective as an antimicrobial compound most in Bacillus subtilis, although some of the dishes showed no area of inhibitions. The disappearance of Bacillus subtilis in most of Petri dishes shown by no growth sign indicated that coriander worked best to inhibit bacteria. This result matched with the previous literature stating that coriander inhibits food pathogenic bacteria, especially Gram-positive bacteria. However, coriander has the least inhibition activity towards Candida tropicalis and Aspergillus oryzae was found to be less susceptible to the volatile compound in coriander acting as antimicrobial compound. There were almost no area of inhibition found in every petri dish, proving that coriander did not affect mold and yeast. Since the literature stated that coriander has almost no antifungal activity and its activity toward yeast has not been examined, the result matched them. 4. 1. 3 Black Pepper Table 4. 3 Observation result of inhibitory activity by black pepper From the result of the experiment, it can be seen that black pepper is most effective when used against Bacillus subtilis, as many bacteria were inactivated, indicated by the area of clear zone, which in some plates, all of them doesn’t grow at all and so the area of clear zone can’t be measured. Aspergillus oryzae are quite susceptible as well to black pepper’s antimicrobial compound as the area of inhibition overall is large. While the most resistant microorganism from the experiment shown is Candida tropicalis, as the area of inhibition is relatively small. From the theory, black pepper shows antimicrobial activity against bacteria, molds, and yeasts, but the strongest towards bacteria, especially Grampositive bacteria. The result of the experiment matches the theory, as black pepper is very effective in stopping the growth of Bacillus subtilis, which is a Grampositive bacterium. It also inhibited the growth of Candida tropicalis and Aspergillus oryzae, so the result of the experiment matches the theory. 4. 1. 4 Potassium Sorbate Table 4. 4 Observation result of inhibitory activity by potassium sorbate From the result of the experiment, it can be seen that potassium sorbate is very effective in stopping the growth of Bacillus subtilis, as in some plates it inhibited the growth of all the bacteria and so the area of clear zone can’t be measured. Antimicrobial activity can be seen as well towards Candida tropicalis and Aspergillus oryzae. According to the theory, potassium sorbate is very effective when used to inhibit the growth of bacteria, molds, and yeasts. The result of the experiment matches the theory, as potassium sorbate in this experiment is able to stop the growth of Bacillus subtilis, Candida tropicalis, and Aspergillus Oryzae. 4. 2 Microbial Defense Towards Antimicrobial Compounds 4. 2. 1 Bacteria (Bacillus subtilis) Table 4. 4 Table of diameter of inhibition zone of Bacillus subtilis in some condiments From the table 4. 4, it is shown that the most effective condiment in affecting the microbial defense of the Bacillus subtilis for its growth is arlic. Also, the least effective condiment for inhibiting the growth of Bacillus subtilis is coriander. According to the theory, garlic is effective for both Gram-negative and Grampositive bacteria, thus the inhibition zone is larger. Also, coriander is effective in inhibiting Gram-negative bacteria, as in the experiment the bacteria used was Gram-positive bacteri a, thus, coriander is not effective in inhibiting Bacillus subtilis. The experiment result has shown the same result as the theory given. 4. 2. 2 Yeast (Candida tropicalis) Table 4. 5Table of diameter of inhibition zone of Candida tropicalis in some condiments In the table 4. 5, it shown that the most effective condiment in affecting the microbial defense and microbial growth of Candida tropicalis is garlic and the least effective is coriander. According to the theory, garlic is used as antifungal and is the most effective one in inhibiting the growth of bacteria, molds and yeast. Thus, the inhibition zone of garlic is the largest. Also, as coriander is mostly effective in inhibiting Gram-negative bacteria, the rate of inhibition of yeast is lower, so the inhibition zone is smaller. The experiment result has shown the exact result as the theory given. 4. 2. Molds (Aspergillus oryzae) Table 4. 6 Table of diameter of inhibition zone of Aspergillus oryzae in some condiments The experiment result has shown that the most effective condiment towards the inhibition zone in Aspergillus oryzae media is garlic and the least effective condiment is coriander. Referring to the theory, garlic is mostly used as antifungal and the most effective condiment used in the experiment to inhibit the growth of bacteria, mold, and yeast. Also, coriander is least effective in inhibiting the growth of Aspergillus oryzae as coriander is most effective in inhibiting gramnegative bacteria. In conclusion, the result of the experiment shows the same result as the theory given. CHAPTER V CONCLUSION Spices have different antimicrobial compounds able to inhibit the growth of various types of microorganisms. Based on the experiments, garlic, coriander, and black pepper have the most effective activity in inhibiting the growth of bacteria, or in this case Bacillus subtilis. However, coriander was proven to have no antifungal activity by the absence of inhibition zone on the Petri plates with yeast and molds, in this case Candida tropicalis and Aspergillus oryzae. In addition, potassium sorbate is effective to inhibit the growth of all types of micoorganisms in the experiment: bacteria, yeast, and molds. The Bacillus subtilis bacteria, Candida tropicalis yeast, and Aspergillus oryzae molds are most susceptible by the presence of garlic. This means that the garlic has the highest effectiveness in affecting the defense of bacteria, yeast, and molds. In contrast, the growth of bacteria, yeast, and molds is least affected by the presence of coriander. How to cite Food Microbiology, Essay examples

Rles Darwin Essays - Charles Darwin, Coleopterists,

rles Darwin Like many students, Charles Darwin was only intrested in topics that was intresting to him and although his father was a doctor, Darwin was very unintrested in medicine and he also couldnt stand the sight of surgery. He did eventually get a degree in Theology from Cambridge University, Although Theology was a minor intrest to him. What Darwin really liked to do was climb over hills, observe plants and animals, collecting new specimens, studying their structures, and categorizing his findings. In 1831, when Darwin was 22 years old, the British government sent her Majesty's ship The Beagle on a 5 year expedition that would take them along the coastline of South America and then onward around the world. During the trip the Beagle would carry along a naturalist to observe and collect Geological and Biological specimens. Thanks to a recomendation from one of Darwins old college professors, he was offered the position aboard the Beagle. The Beagle sailed to South America, making many stops along the coast. Here Darwin observed the plants and animals of the tropics and was stunned by the diversity of species compared with Europe. The most significant stop the Beagle made was the Galapagos Islands off the northwestern coast of South America. It was here that Darwin found huge populations of Tortoises; and he found out that diffrent islands were home to diffrent types of tortoises. He found that islands without tortoises, pricky pear cactus plants grew with their fruits spread all over the ground. And on Islands that had lots of tortoises, the prickly pears grew really thick, tall, bearing the fruit high above the tortoises reach. He wondered if the differences in the two plants were from being isolated from one another on seperate islands. In 1836, Darwin returned to England after his 5 year expedition. He became established as one of the best naturalists of his time. But Darwin sought to prove his idea of evolution with simple examples. Darwin maintained that seperate species evolve as a result of Natural Selection, or survival of the fittest. Darwin never said that human beings evolved from apes. He said that all life began with molecules acting on each other. So from the first single celled organism all life came. One single organism, by many diffrent molecules could make many diffrent species of animals. It was in this way that he stated Ape and Man are similar by each having similar life beginning. Darwin's theories caused people to begin to question where they actually came from. His response was the book on the origin of species. In his book he addressed the concerns of the people. He said It is interesting to contemplate an entangled bank, clothed with many plants of many kinds, with birds singing in the bushes, with various insects flitting about, and with worms crawling through the damp earth, and to reflect that these elaborately constructed forms have all been produced by laws acting around us. These laws, taken in the highes sense, being growth with reproduction; Inheritance and Variability; a Ratio of Increase so high as to lead to a strugle for life, and as a consequence to Natural Selection, entailing Divergence of charector and extinction of less-improved forms. There is grandeur in this view of life, with its several powers, having been originally breathed into a few forms or into one, and that, whilst this planet has gone cycling on according to the fixed laws of gravity, from so simple a beginning endless forms most beauthiful and most wonderful have been, and are being, evolved. By, Mike M